Virus:Article Title: Single-Unit Imaging of Membrane Protein-Embedded Nanodiscs from Two Oriented Sides by High-Speed Atomic Force Microscopy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains BL21(DE3) Laboratory stock N/A Rosetta2(DE3) Novagen Cat# 71397 Chemicals, Peptides, and Recombinant Proteins n-dodecyl-b-D-maltopyranoside (DDM) Glycon Cat# D97002 Biotin-PEAC5-maleimide Dojindo Cat# 344-06391 MSP1D1 Laboratory stock N/A E. coli Total Lipid Extract Avanti Polar Lipids Cat# 100500 Bio-Beads SM-2 Adsorbents BIO-RAD Cat# 1528920 TEV protease Laboratory stock N/A Recombinant DNA Plasmid : Vector pTV118N Takara Shuzo Cat# 3328 Plasmid : Vector pACYCDuet-1 Novagen Cat# 71147-3 Plasmid : Vector pET-16b Daimon et al., 2017 N/A pTT610 (pTV118N-based vector) Addgene Cat# 101742 pAK24 (pACYCDuet-1-based vector) Addgene Cat# 101741 T. thermophilus mgtE RIKEN BRC Cat# JCM 10941 Software and Algorithms Kodec 4.5.7.22 (for cross-sectional analysis) Kodera et al., 2010 N/A Origin 2018 (for a graph creation and Gaussian fitting) OriginLab https://www.originlab.com/ Pymol 2.0 (to draw a model of membrane protein-embedded in nanodisc) https://www.pymol.org/ N/A IgorPro 6.36 (for high-speed AFM analysis) WaveMetrics https://www.wavemetrics.com/ Deposited Data SecYEG crystal structure Tanaka et al., 2015 PDB: 5AWW SecA-SecYEG crystal structure Zimmer et al., 2008 PDB: 3DIN Model of nanodisc including membrane scaffold proteins and lipids Mazhab-Jafari et al., 2015 PDB: 2MSC MgtE crystal structure Hattori et al., 2007 PDB: 2YVX Other Laboratory-built high-speed AFM Ando et al., 2001, 2008; Uchihashi et al., 2012 N/A .. Each E. coli transformant altered from the E. coli strains BL21 (DE3) (Laboratory stock) and Rosetta 2(DE3) (Novagen, Darmstadt, Germany) was isolated and stored in LB media containing 20% glycerol at 80 C as a glycerol stock.
Recombinant:Article Title: Single-Unit Imaging of Membrane Protein-Embedded Nanodiscs from Two Oriented Sides by High-Speed Atomic Force Microscopy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains BL21(DE3) Laboratory stock N/A Rosetta2(DE3) Novagen Cat# 71397 Chemicals, Peptides, and Recombinant Proteins n-dodecyl-b-D-maltopyranoside (DDM) Glycon Cat# D97002 Biotin-PEAC5-maleimide Dojindo Cat# 344-06391 MSP1D1 Laboratory stock N/A E. coli Total Lipid Extract Avanti Polar Lipids Cat# 100500 Bio-Beads SM-2 Adsorbents BIO-RAD Cat# 1528920 TEV protease Laboratory stock N/A Recombinant DNA Plasmid : Vector pTV118N Takara Shuzo Cat# 3328 Plasmid : Vector pACYCDuet-1 Novagen Cat# 71147-3 Plasmid : Vector pET-16b Daimon et al., 2017 N/A pTT610 (pTV118N-based vector) Addgene Cat# 101742 pAK24 (pACYCDuet-1-based vector) Addgene Cat# 101741 T. thermophilus mgtE RIKEN BRC Cat# JCM 10941 Software and Algorithms Kodec 4.5.7.22 (for cross-sectional analysis) Kodera et al., 2010 N/A Origin 2018 (for a graph creation and Gaussian fitting) OriginLab https://www.originlab.com/ Pymol 2.0 (to draw a model of membrane protein-embedded in nanodisc) https://www.pymol.org/ N/A IgorPro 6.36 (for high-speed AFM analysis) WaveMetrics https://www.wavemetrics.com/ Deposited Data SecYEG crystal structure Tanaka et al., 2015 PDB: 5AWW SecA-SecYEG crystal structure Zimmer et al., 2008 PDB: 3DIN Model of nanodisc including membrane scaffold proteins and lipids Mazhab-Jafari et al., 2015 PDB: 2MSC MgtE crystal structure Hattori et al., 2007 PDB: 2YVX Other Laboratory-built high-speed AFM Ando et al., 2001, 2008; Uchihashi et al., 2012 N/A .. Each E. coli transformant altered from the E. coli strains BL21 (DE3) (Laboratory stock) and Rosetta 2(DE3) (Novagen, Darmstadt, Germany) was isolated and stored in LB media containing 20% glycerol at 80 C as a glycerol stock.
Plasmid Preparation:Article Title: Single-Unit Imaging of Membrane Protein-Embedded Nanodiscs from Two Oriented Sides by High-Speed Atomic Force Microscopy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains BL21(DE3) Laboratory stock N/A Rosetta2(DE3) Novagen Cat# 71397 Chemicals, Peptides, and Recombinant Proteins n-dodecyl-b-D-maltopyranoside (DDM) Glycon Cat# D97002 Biotin-PEAC5-maleimide Dojindo Cat# 344-06391 MSP1D1 Laboratory stock N/A E. coli Total Lipid Extract Avanti Polar Lipids Cat# 100500 Bio-Beads SM-2 Adsorbents BIO-RAD Cat# 1528920 TEV protease Laboratory stock N/A Recombinant DNA Plasmid : Vector pTV118N Takara Shuzo Cat# 3328 Plasmid : Vector pACYCDuet-1 Novagen Cat# 71147-3 Plasmid : Vector pET-16b Daimon et al., 2017 N/A pTT610 (pTV118N-based vector) Addgene Cat# 101742 pAK24 (pACYCDuet-1-based vector) Addgene Cat# 101741 T. thermophilus mgtE RIKEN BRC Cat# JCM 10941 Software and Algorithms Kodec 4.5.7.22 (for cross-sectional analysis) Kodera et al., 2010 N/A Origin 2018 (for a graph creation and Gaussian fitting) OriginLab https://www.originlab.com/ Pymol 2.0 (to draw a model of membrane protein-embedded in nanodisc) https://www.pymol.org/ N/A IgorPro 6.36 (for high-speed AFM analysis) WaveMetrics https://www.wavemetrics.com/ Deposited Data SecYEG crystal structure Tanaka et al., 2015 PDB: 5AWW SecA-SecYEG crystal structure Zimmer et al., 2008 PDB: 3DIN Model of nanodisc including membrane scaffold proteins and lipids Mazhab-Jafari et al., 2015 PDB: 2MSC MgtE crystal structure Hattori et al., 2007 PDB: 2YVX Other Laboratory-built high-speed AFM Ando et al., 2001, 2008; Uchihashi et al., 2012 N/A .. Each E. coli transformant altered from the E. coli strains BL21 (DE3) (Laboratory stock) and Rosetta 2(DE3) (Novagen, Darmstadt, Germany) was isolated and stored in LB media containing 20% glycerol at 80 C as a glycerol stock.
Software:Article Title: Single-Unit Imaging of Membrane Protein-Embedded Nanodiscs from Two Oriented Sides by High-Speed Atomic Force Microscopy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains BL21(DE3) Laboratory stock N/A Rosetta2(DE3) Novagen Cat# 71397 Chemicals, Peptides, and Recombinant Proteins n-dodecyl-b-D-maltopyranoside (DDM) Glycon Cat# D97002 Biotin-PEAC5-maleimide Dojindo Cat# 344-06391 MSP1D1 Laboratory stock N/A E. coli Total Lipid Extract Avanti Polar Lipids Cat# 100500 Bio-Beads SM-2 Adsorbents BIO-RAD Cat# 1528920 TEV protease Laboratory stock N/A Recombinant DNA Plasmid : Vector pTV118N Takara Shuzo Cat# 3328 Plasmid : Vector pACYCDuet-1 Novagen Cat# 71147-3 Plasmid : Vector pET-16b Daimon et al., 2017 N/A pTT610 (pTV118N-based vector) Addgene Cat# 101742 pAK24 (pACYCDuet-1-based vector) Addgene Cat# 101741 T. thermophilus mgtE RIKEN BRC Cat# JCM 10941 Software and Algorithms Kodec 4.5.7.22 (for cross-sectional analysis) Kodera et al., 2010 N/A Origin 2018 (for a graph creation and Gaussian fitting) OriginLab https://www.originlab.com/ Pymol 2.0 (to draw a model of membrane protein-embedded in nanodisc) https://www.pymol.org/ N/A IgorPro 6.36 (for high-speed AFM analysis) WaveMetrics https://www.wavemetrics.com/ Deposited Data SecYEG crystal structure Tanaka et al., 2015 PDB: 5AWW SecA-SecYEG crystal structure Zimmer et al., 2008 PDB: 3DIN Model of nanodisc including membrane scaffold proteins and lipids Mazhab-Jafari et al., 2015 PDB: 2MSC MgtE crystal structure Hattori et al., 2007 PDB: 2YVX Other Laboratory-built high-speed AFM Ando et al., 2001, 2008; Uchihashi et al., 2012 N/A .. Each E. coli transformant altered from the E. coli strains BL21 (DE3) (Laboratory stock) and Rosetta 2(DE3) (Novagen, Darmstadt, Germany) was isolated and stored in LB media containing 20% glycerol at 80 C as a glycerol stock.
Membrane:Article Title: Single-Unit Imaging of Membrane Protein-Embedded Nanodiscs from Two Oriented Sides by High-Speed Atomic Force Microscopy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains BL21(DE3) Laboratory stock N/A Rosetta2(DE3) Novagen Cat# 71397 Chemicals, Peptides, and Recombinant Proteins n-dodecyl-b-D-maltopyranoside (DDM) Glycon Cat# D97002 Biotin-PEAC5-maleimide Dojindo Cat# 344-06391 MSP1D1 Laboratory stock N/A E. coli Total Lipid Extract Avanti Polar Lipids Cat# 100500 Bio-Beads SM-2 Adsorbents BIO-RAD Cat# 1528920 TEV protease Laboratory stock N/A Recombinant DNA Plasmid : Vector pTV118N Takara Shuzo Cat# 3328 Plasmid : Vector pACYCDuet-1 Novagen Cat# 71147-3 Plasmid : Vector pET-16b Daimon et al., 2017 N/A pTT610 (pTV118N-based vector) Addgene Cat# 101742 pAK24 (pACYCDuet-1-based vector) Addgene Cat# 101741 T. thermophilus mgtE RIKEN BRC Cat# JCM 10941 Software and Algorithms Kodec 4.5.7.22 (for cross-sectional analysis) Kodera et al., 2010 N/A Origin 2018 (for a graph creation and Gaussian fitting) OriginLab https://www.originlab.com/ Pymol 2.0 (to draw a model of membrane protein-embedded in nanodisc) https://www.pymol.org/ N/A IgorPro 6.36 (for high-speed AFM analysis) WaveMetrics https://www.wavemetrics.com/ Deposited Data SecYEG crystal structure Tanaka et al., 2015 PDB: 5AWW SecA-SecYEG crystal structure Zimmer et al., 2008 PDB: 3DIN Model of nanodisc including membrane scaffold proteins and lipids Mazhab-Jafari et al., 2015 PDB: 2MSC MgtE crystal structure Hattori et al., 2007 PDB: 2YVX Other Laboratory-built high-speed AFM Ando et al., 2001, 2008; Uchihashi et al., 2012 N/A .. Each E. coli transformant altered from the E. coli strains BL21 (DE3) (Laboratory stock) and Rosetta 2(DE3) (Novagen, Darmstadt, Germany) was isolated and stored in LB media containing 20% glycerol at 80 C as a glycerol stock.
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